GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).[supplied by OMIM, Aug 2009]
Transcription factors with Perturb-seq knockdown data for GMDS. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GMDS upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GMDS, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:1,595,602–1,596,723 | 649.4 kb | Distal (>10kb) Multiome HiCAR | 325 | |
| chr6:1,596,826–1,597,922 | 648.2 kb | Distal (>10kb) Multiome HiCAR | 307 | |
| chr6:1,604,125–1,605,503 | 641.1 kb | Distal (>10kb) Multiome HiCAR | 591 | |
| chr6:1,606,436–1,607,329 | 638.7 kb | Distal (>10kb) Multiome HiCAR | 251 | |
| chr6:1,607,558–1,608,252 | 637.7 kb | Distal (>10kb) Multiome HiCAR | 448 | |
| chr6:1,608,357–1,612,111 | 635.8 kb | Distal (>10kb) Multiome HiCAR | 614 | |
| chr6:1,613,539–1,615,618 | 9.1 kb | Proximal (<10kb) | 556 | |
| chr6:1,619,218–1,620,936 | 3.8 kb | Proximal (<10kb) | 223 | |
| chr6:1,623,948–1,625,142 | at TSS | At TSS | 256 | |
| chr6:1,627,964–1,628,409 | 3.2 kb | Proximal (<10kb) | 70 | |
| chr6:1,720,613–1,721,074 | 5.4 kb | Proximal (<10kb) | 97 | |
| chr6:1,724,222–1,724,530 | 1.9 kb | Proximal (<10kb) | 87 | |
| chr6:1,724,679–1,724,960 | 1.5 kb | Proximal (<10kb) | 156 | |
| chr6:1,726,266–1,726,743 | at TSS | At TSS | 172 | |
| chr6:1,729,954–1,730,748 | 3.5 kb | Proximal (<10kb) | 55 | |
| chr6:2,244,856–2,246,522 | 27 bp | At TSS Multiome | 1006 |
Genomic view of the GMDS locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.