The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for GGCT. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = GGCT upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of GGCT, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:30,284,014–30,286,043 | 220.3 kb | Distal (>10kb) Multiome | 943 | |
| chr7:30,472,499–30,473,444 | 31.9 kb | Distal (>10kb) Multiome | 123 | |
| chr7:30,478,426–30,479,128 | 26.2 kb | Distal (>10kb) Multiome | 756 | |
| chr7:30,504,202–30,505,455 | 82 bp | At TSS Multiome | 824 | |
| chr7:30,544,196–30,544,698 | 39.7 kb | Distal (>10kb) Multiome | 40 | |
| chr7:30,594,119–30,595,511 | 90.0 kb | Distal (>10kb) Multiome | 1016 | |
| chr7:30,681,420–30,682,927 | 177.7 kb | Distal (>10kb) Multiome | 319 | |
| chr7:30,699,970–30,700,584 | 195.5 kb | Distal (>10kb) Multiome | 149 | |
| chr7:30,771,029–30,771,985 | 266.6 kb | Distal (>10kb) Multiome | 536 |
Genomic view of the GGCT locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.