Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for FST. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FST upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FST, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:53,432,594–53,434,095 | 47.2 kb | Distal (>10kb) Multiome | 196 | |
| chr5:53,478,197–53,479,326 | 1.9 kb | Proximal (<10kb) Multiome | 625 | |
| chr5:53,479,490–53,481,204 | 259 bp | At TSS Multiome | 549 | |
| chr5:53,481,901–53,482,204 | 1.3 kb | Proximal (<10kb) | 451 | |
| chr5:53,482,527–53,483,492 | 2.4 kb | Proximal (<10kb) Multiome | 564 | |
| chr5:53,488,242–53,488,597 | 7.6 kb | Proximal (<10kb) | 34 | |
| chr5:53,552,743–53,553,747 | 72.5 kb | Distal (>10kb) Multiome | 169 | |
| chr5:53,560,372–53,561,195 | 80.2 kb | Distal (>10kb) Multiome | 824 | |
| chr5:53,671,258–53,672,124 | 191.0 kb | Distal (>10kb) Multiome | 162 | |
| chr5:53,997,875–53,998,940 | 517.7 kb | Distal (>10kb) Multiome HiCAR | 119 |
Genomic view of the FST locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.