This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for FBXW8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FBXW8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FBXW8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:116,694,557–116,695,015 | 216.2 kb | Distal (>10kb) Multiome | 37 | |
| chr12:116,697,179–116,697,642 | 213.5 kb | Distal (>10kb) Multiome | 129 | |
| chr12:116,737,743–116,738,634 | 172.8 kb | Distal (>10kb) Multiome | 852 | |
| chr12:116,818,976–116,819,922 | 91.4 kb | Distal (>10kb) Multiome | 760 | |
| chr12:116,876,126–116,876,628 | 34.4 kb | Distal (>10kb) Multiome | 82 | |
| chr12:116,880,733–116,881,665 | 29.8 kb | Distal (>10kb) Multiome HiCAR | 509 | |
| chr12:116,910,746–116,911,448 | 87 bp | At TSS Multiome | 902 | |
| chr12:116,918,256–116,918,760 | 7.3 kb | Proximal (<10kb) | 79 | |
| chr12:116,919,523–116,919,693 | 8.6 kb | Proximal (<10kb) | 207 | |
| chr12:117,051,045–117,051,880 | 140.6 kb | Distal (>10kb) Multiome | 103 | |
| chr12:117,052,572–117,053,115 | 142.0 kb | Distal (>10kb) Multiome | 88 | |
| chr12:117,098,887–117,099,725 | 188.4 kb | Distal (>10kb) Multiome | 608 | |
| chr12:117,118,728–117,119,232 | 207.9 kb | Distal (>10kb) Multiome | 141 | |
| chr12:117,155,214–117,156,022 | 244.5 kb | Distal (>10kb) Multiome | 507 | |
| chr12:117,189,825–117,190,955 | 279.5 kb | Distal (>10kb) Multiome | 751 |
Genomic view of the FBXW8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.