Members of the F-box protein family, such as FBXO28, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for FBXO28. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FBXO28 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FBXO28, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:223,845,336–223,846,577 | 268.1 kb | Distal (>10kb) Multiome | 1001 | |
| chr1:223,870,523–223,871,422 | 243.3 kb | Distal (>10kb) Multiome | 131 | |
| chr1:223,992,339–223,993,214 | 121.4 kb | Distal (>10kb) Multiome | 350 | |
| chr1:224,113,911–224,114,795 | 19 bp | At TSS Multiome | 853 | |
| chr1:224,182,841–224,184,249 | 69.1 kb | Distal (>10kb) Multiome | 901 | |
| chr1:224,329,851–224,330,705 | 216.2 kb | Distal (>10kb) Multiome | 865 | |
| chr1:224,356,549–224,357,612 | 242.8 kb | Distal (>10kb) Multiome | 791 | |
| chr1:224,929,306–224,930,138 | 815.5 kb | Distal (>10kb) Multiome HiCAR | 992 |
Genomic view of the FBXO28 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.