This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains 12 tandem leucine-rich repeats. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]
Transcription factors with Perturb-seq knockdown data for FBXL2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FBXL2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FBXL2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:32,998,701–32,999,462 | 278.4 kb | Distal (>10kb) Multiome | 41 | |
| chr3:33,061,228–33,062,289 | 215.7 kb | Distal (>10kb) Multiome | 238 | |
| chr3:33,096,220–33,097,726 | 180.2 kb | Distal (>10kb) Multiome | 905 | |
| chr3:33,113,579–33,114,881 | 163.1 kb | Distal (>10kb) Multiome | 870 | |
| chr3:33,218,068–33,219,328 | 58.6 kb | Distal (>10kb) Multiome | 329 | |
| chr3:33,277,037–33,277,887 | 10 bp | At TSS Multiome | 534 | |
| chr3:33,278,204–33,278,664 | 739 bp | At TSS | 102 | |
| chr3:33,425,913–33,426,646 | 148.8 kb | Distal (>10kb) Multiome | 224 | |
| chr3:33,439,404–33,441,603 | 163.1 kb | Distal (>10kb) Multiome | 1014 | |
| chr3:33,446,268–33,447,578 | 169.1 kb | Distal (>10kb) Multiome | 160 |
Genomic view of the FBXL2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.