Transcription factors with Perturb-seq knockdown data for FAM222A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = FAM222A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of FAM222A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:109,476,943–109,477,929 | 236.4 kb | Distal (>10kb) Multiome | 911 | |
| chr12:109,573,025–109,574,078 | 140.2 kb | Distal (>10kb) Multiome HiCAR | 1005 | |
| chr12:109,597,611–109,598,240 | 115.9 kb | Distal (>10kb) Multiome HiCAR | 536 | |
| chr12:109,679,688–109,680,306 | 33.7 kb | Distal (>10kb) Multiome | 69 | |
| chr12:109,697,996–109,698,635 | 15.5 kb | Distal (>10kb) Multiome HiCAR | 115 | |
| chr12:109,713,288–109,715,156 | 97 bp | At TSS Multiome | 793 | |
| chr12:109,715,315–109,715,901 | 1.5 kb | Proximal (<10kb) | 125 | |
| chr12:109,717,933–109,718,896 | 4.7 kb | Proximal (<10kb) Multiome | 199 | |
| chr12:109,719,473–109,719,619 | 5.6 kb | Proximal (<10kb) | 105 | |
| chr12:109,737,442–109,738,212 | 24.1 kb | Distal (>10kb) Multiome HiCAR | 231 | |
| chr12:109,739,699–109,741,057 | 26.4 kb | Distal (>10kb) Multiome HiCAR | 245 | |
| chr12:109,833,102–109,833,778 | 119.6 kb | Distal (>10kb) Multiome | 217 | |
| chr12:109,845,155–109,845,762 | 131.6 kb | Distal (>10kb) Multiome | 618 | |
| chr12:109,880,019–109,881,173 | 166.8 kb | Distal (>10kb) Multiome | 576 | |
| chr12:109,900,106–109,900,747 | 186.5 kb | Distal (>10kb) Multiome | 818 | |
| chr12:109,995,726–109,996,660 | 282.5 kb | Distal (>10kb) Multiome | 811 | |
| chr12:109,998,999–109,999,838 | 285.4 kb | Distal (>10kb) Multiome | 883 |
Genomic view of the FAM222A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.