This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Aug 2020]
Transcription factors with Perturb-seq knockdown data for F3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = F3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of F3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:94,247,152–94,248,342 | 294.0 kb | Distal (>10kb) Multiome | 521 | |
| chr1:94,327,061–94,327,698 | 214.2 kb | Distal (>10kb) Multiome | 243 | |
| chr1:94,342,282–94,342,957 | 199.1 kb | Distal (>10kb) Multiome | 83 | |
| chr1:94,417,844–94,419,337 | 123.4 kb | Distal (>10kb) Multiome | 948 | |
| chr1:94,538,349–94,538,533 | 3.2 kb | Proximal (<10kb) | 38 | |
| chr1:94,540,914–94,542,577 | 87 bp | At TSS Multiome | 726 | |
| chr1:94,544,799–94,544,931 | 3.0 kb | Proximal (<10kb) | 53 | |
| chr1:94,570,815–94,571,430 | 29.4 kb | Distal (>10kb) Multiome | 129 | |
| chr1:94,572,275–94,573,057 | 30.9 kb | Distal (>10kb) Multiome | 73 | |
| chr1:94,657,564–94,658,605 | 116.4 kb | Distal (>10kb) Multiome | 208 | |
| chr1:94,819,759–94,820,829 | 278.6 kb | Distal (>10kb) Multiome HiCAR | 576 |
Genomic view of the F3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.