This gene encodes a member of the apyrase protein family. Apyrases are enzymes that catalyze the hydrolysis of nucleotide diphosphates and triphosphates in a calcium or magnesium-dependent manner. The encoded protein is an endo-apyrase and may play a role in salvaging nucleotides from lysosomes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and these isoforms may differ in divalent cation dependence and substrate specificity. [provided by RefSeq, Sep 2011]
Transcription factors with Perturb-seq knockdown data for ENTPD4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ENTPD4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ENTPD4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:23,162,920–23,164,386 | 293.7 kb | Distal (>10kb) Multiome | 808 | |
| chr8:23,224,394–23,225,450 | 232.6 kb | Distal (>10kb) Multiome | 678 | |
| chr8:23,246,206–23,247,324 | 211.0 kb | Distal (>10kb) Multiome | 941 | |
| chr8:23,287,908–23,288,715 | 169.5 kb | Distal (>10kb) Multiome | 853 | |
| chr8:23,403,157–23,405,109 | 53.5 kb | Distal (>10kb) Multiome | 425 | |
| chr8:23,451,094–23,451,922 | 6.2 kb | Proximal (<10kb) Multiome | 182 | |
| chr8:23,452,593–23,453,000 | 4.6 kb | Proximal (<10kb) | 250 | |
| chr8:23,457,148–23,458,219 | 38 bp | At TSS Multiome | 736 | |
| chr8:23,486,400–23,487,438 | 29.2 kb | Distal (>10kb) Multiome | 134 | |
| chr8:23,528,169–23,530,101 | 71.2 kb | Distal (>10kb) Multiome | 832 | |
| chr8:23,682,166–23,683,403 | 225.3 kb | Distal (>10kb) Multiome | 655 | |
| chr8:23,706,237–23,707,411 | 249.2 kb | Distal (>10kb) Multiome HiCAR | 215 | |
| chr8:23,709,392–23,710,292 | 252.3 kb | Distal (>10kb) Multiome | 98 | |
| chr8:23,714,334–23,715,166 | 257.1 kb | Distal (>10kb) Multiome HiCAR | 334 | |
| chr8:24,414,847–24,415,546 | 957.6 kb | Distal (>10kb) Multiome HiCAR | 251 |
Genomic view of the ENTPD4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.