Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]
Transcription factors with Perturb-seq knockdown data for EIF6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EIF6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EIF6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr20:35,092,320–35,093,398 | 192.0 kb | Distal (>10kb) Multiome | 1139 | |
| chr20:35,146,563–35,147,734 | 137.5 kb | Distal (>10kb) Multiome | 992 | |
| chr20:35,171,731–35,172,358 | 112.8 kb | Distal (>10kb) Multiome | 383 | |
| chr20:35,174,680–35,175,277 | 109.8 kb | Distal (>10kb) Multiome | 635 | |
| chr20:35,262,839–35,263,582 | 21.4 kb | Distal (>10kb) Multiome | 336 | |
| chr20:35,269,197–35,269,766 | 15.3 kb | Distal (>10kb) Multiome | 447 | |
| chr20:35,277,415–35,278,519 | 6.7 kb | Proximal (<10kb) Multiome | 575 | |
| chr20:35,284,235–35,285,360 | 11 bp | At TSS Multiome | 950 | |
| chr20:35,303,859–35,304,479 | 19.3 kb | Distal (>10kb) Multiome | 484 | |
| chr20:35,454,521–35,455,845 | 170.4 kb | Distal (>10kb) Multiome | 1059 | |
| chr20:35,539,672–35,540,321 | 255.2 kb | Distal (>10kb) Multiome | 311 | |
| chr20:35,541,280–35,542,685 | 257.3 kb | Distal (>10kb) Multiome | 849 |
Genomic view of the EIF6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.