The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Transcription factors with Perturb-seq knockdown data for EHHADH. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EHHADH upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EHHADH, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:185,152,390–185,153,792 | 101.2 kb | Distal (>10kb) Multiome | 589 | |
| chr3:185,249,926–185,250,485 | 3.9 kb | Proximal (<10kb) Multiome | 51 | |
| chr3:185,253,856–185,254,366 | at TSS | At TSS | 650 | |
| chr3:185,267,961–185,268,947 | 14.5 kb | Distal (>10kb) Multiome | 258 | |
| chr3:185,269,944–185,270,809 | 16.5 kb | Distal (>10kb) Multiome | 180 | |
| chr3:185,282,769–185,283,294 | 28.9 kb | Distal (>10kb) Multiome | 660 | |
| chr3:185,372,493–185,373,188 | 118.7 kb | Distal (>10kb) Multiome | 60 | |
| chr3:185,498,399–185,499,479 | 245.0 kb | Distal (>10kb) Multiome | 1024 |
Genomic view of the EHHADH locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.