ECT2
epithelial cell transforming 2 | ARHGEF31

The protein encoded by this gene is a guanine nucleotide exchange factor and transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2017]

Member of: DE-11 DE-11.1 Developmental clusters: GC5
Biological processes 58 terms
GTPase activator activity (GO:0005096)GTPase activator activity (GO:0005096)activation of GTPase activity (GO:0090630)activation of GTPase activity (GO:0090630)activation of protein kinase activity (GO:0032147)anchoring junction (GO:0070161)bicellular tight junction (GO:0005923)bicellular tight junction (GO:0005923)bicellular tight junction assembly (GO:0070830)cell cortex (GO:0005938)cell-cell junction (GO:0005911)cellular response to calcium ion (GO:0071277)cellular response to hydrogen peroxide (GO:0070301)cellular response to ionizing radiation (GO:0071479)centralspindlin complex (GO:0097149)centrosome (GO:0005813)cleavage furrow (GO:0032154)cleavage furrow (GO:0032154)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytosol (GO:0005829)cytosol (GO:0005829)guanyl-nucleotide exchange factor activity (GO:0005085)guanyl-nucleotide exchange factor activity (GO:0005085)guanyl-nucleotide exchange factor activity (GO:0005085)guanyl-nucleotide exchange factor activity (GO:0005085)intracellular signal transduction (GO:0035556)midbody (GO:0030496)midbody (GO:0030496)mitotic cytokinesis (GO:0000281)mitotic cytokinesis (GO:0000281)mitotic spindle (GO:0072686)nervous system development (GO:0007399)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of GTPase activity (GO:0043547)positive regulation of GTPase activity (GO:0043547)positive regulation of apoptotic process (GO:0043065)positive regulation of canonical NF-kappaB signal transduction (GO:0043123)positive regulation of cytokinesis (GO:0032467)positive regulation of cytokinesis (GO:0032467)positive regulation of mitotic cytokinetic process (GO:1903438)positive regulation of neuron differentiation (GO:0045666)positive regulation of neuron differentiation (GO:0045666)positive regulation of protein import into nucleus (GO:0042307)positive regulation of protein import into nucleus (GO:0042307)protein binding (GO:0005515)protein homodimerization activity (GO:0042803)protein homooligomerization (GO:0051260)regulation of attachment of spindle microtubules to kinetochore (GO:0051988)regulation of cytokinesis, actomyosin contractile ring assembly (GO:2000431)regulation of protein kinase activity (GO:0045859)regulation of small GTPase mediated signal transduction (GO:0051056)small GTPase binding (GO:0031267)spindle (GO:0005819)
Expression (TPM)
ECT2 — as a Regulated Gene

TFs regulating ECT2 0 TFs

Transcription factors with Perturb-seq knockdown data for ECT2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ECT2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

Data: Effect:
TF Mean coef Binding Outlier TF→Gene link

Elements linked to ECT2

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ECT2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Accessibility Element Dist. to TSS Link type TFs
chr3:172,607,503–172,608,119 142.8 kb Distal (>10kb) Multiome 194
chr3:172,666,339–172,667,295 83.8 kb Distal (>10kb) Multiome 304
chr3:172,710,050–172,711,735 39.6 kb Distal (>10kb) Multiome 856
chr3:172,750,131–172,751,448 72 bp At TSS Multiome 809

Genome Browser

Genomic view of the ECT2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.

chr3:172,597,503 – 172,761,448
Proximal 1 kb Distal 10 kb Multiome HiCAR ATAC-seq RNA-seq