This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ECH1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ECH1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ECH1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr19:38,565,090–38,565,701 | 266.4 kb | Distal (>10kb) Multiome | 164 | |
| chr19:38,595,857–38,597,322 | 235.4 kb | Distal (>10kb) Multiome | 478 | |
| chr19:38,617,752–38,619,569 | 212.8 kb | Distal (>10kb) Multiome | 835 | |
| chr19:38,647,137–38,648,653 | 184.3 kb | Distal (>10kb) Multiome | 856 | |
| chr19:38,666,103–38,666,668 | 165.5 kb | Distal (>10kb) Multiome | 317 | |
| chr19:38,683,677–38,684,144 | 148.1 kb | Distal (>10kb) Multiome | 678 | |
| chr19:38,734,491–38,735,702 | 96.5 kb | Distal (>10kb) Multiome | 338 | |
| chr19:38,735,981–38,737,634 | 94.8 kb | Distal (>10kb) Multiome HiCAR | 493 | |
| chr19:38,754,913–38,755,455 | 76.6 kb | Distal (>10kb) Multiome | 12 | |
| chr19:38,831,191–38,832,119 | 95 bp | At TSS Multiome | 931 | |
| chr19:38,849,166–38,852,789 | 20.3 kb | Distal (>10kb) Multiome | 1139 | |
| chr19:38,869,729–38,870,536 | 38.2 kb | Distal (>10kb) Multiome | 350 | |
| chr19:38,899,267–38,900,530 | 68.1 kb | Distal (>10kb) Multiome | 886 | |
| chr19:38,930,044–38,931,363 | 98.9 kb | Distal (>10kb) Multiome | 889 | |
| chr19:38,949,549–38,950,582 | 118.1 kb | Distal (>10kb) Multiome | 330 | |
| chr19:38,975,192–38,976,859 | 144.9 kb | Distal (>10kb) Multiome | 846 | |
| chr19:38,995,916–38,996,388 | 164.4 kb | Distal (>10kb) Multiome | 144 | |
| chr19:39,030,958–39,033,042 | 199.8 kb | Distal (>10kb) Multiome | 763 | |
| chr19:39,125,485–39,126,290 | 294.0 kb | Distal (>10kb) Multiome | 866 |
Genomic view of the ECH1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.