EBF4

EBF transcription factor 4 | COE4, KIAA1442, O/E-4, RP5-860F19.3

EBF4 belongs to the conserved Olf/EBF family of helix-loop-helix transcription factors, members of which play important roles in neural development and B-cell maturation (Wang et al., 2002 [PubMed 12139918]).[supplied by OMIM, Mar 2008]

Developmental clusters: GC6

Biological processes 21 terms

DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor binding (GO:0140297)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II transcription regulatory region sequence-specific DNA binding (GO:0000977)T cell apoptotic process (GO:0070231)chromatin (GO:0000785)chromatin (GO:0000785)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)protein binding (GO:0005515)regulation of DNA-templated transcription (GO:0006355)regulation of transcription by RNA polymerase II (GO:0006357)

Expression (TPM)

TFs regulating EBF4 0 TFs

Transcription factors with Perturb-seq knockdown data for EBF4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = EBF4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to EBF4

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of EBF4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr20:2,470,152–2,471,296	221.9 kb	Distal (>10kb) Multiome	1126
chr20:2,508,648–2,509,663	183.7 kb	Distal (>10kb) Multiome	854
chr20:2,524,432–2,525,455	168.1 kb	Distal (>10kb) Multiome	724
chr20:2,536,033–2,536,583	156.5 kb	Distal (>10kb) Multiome	42
chr20:2,651,802–2,653,286	40.2 kb	Distal (>10kb) Multiome	1060
chr20:2,663,925–2,664,409	28.5 kb	Distal (>10kb) Multiome	908
chr20:2,688,083–2,688,256	4.5 kb	Proximal (<10kb)	212
chr20:2,692,159–2,694,013	164 bp	At TSS Multiome	584
chr20:2,701,838–2,702,067	9.1 kb	Proximal (<10kb)	47
chr20:2,800,326–2,801,021	107.9 kb	Distal (>10kb) Multiome	349
chr20:2,840,006–2,841,330	147.9 kb	Distal (>10kb) Multiome	997
chr20:2,872,329–2,874,047	180.8 kb	Distal (>10kb) Multiome	980

Genome Browser

Genomic view of the EBF4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.