E2F4

E2F transcription factor 4 | E2F-4

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer. [provided by RefSeq, Jul 2008]

Member of: DE-1 DE-1.14

Biological processes 37 terms

DNA binding (GO:0003677)DNA binding (GO:0003677)DNA-binding transcription activator activity, RNA polymerase II-specific (GO:0001228)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity (GO:0003700)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)DNA-binding transcription factor activity, RNA polymerase II-specific (GO:0000981)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II cis-regulatory region sequence-specific DNA binding (GO:0000978)RNA polymerase II transcription regulator complex (GO:0090575)centriole assembly (GO:0098534)chromatin (GO:0000785)chromatin (GO:0000785)chromatin (GO:0000785)chromatin binding (GO:0003682)cytoplasm (GO:0005737)motile cilium assembly (GO:0044458)multi-ciliated epithelial cell differentiation (GO:1903251)negative regulation of DNA-templated transcription (GO:0045892)nucleoplasm (GO:0005654)nucleoplasm (GO:0005654)nucleus (GO:0005634)nucleus (GO:0005634)positive regulation of transcription by RNA polymerase II (GO:0045944)positive regulation of transcription by RNA polymerase II (GO:0045944)promoter-specific chromatin binding (GO:1990841)protein binding (GO:0005515)protein dimerization activity (GO:0046983)protein domain specific binding (GO:0019904)regulation of DNA-templated transcription (GO:0006355)regulation of DNA-templated transcription (GO:0006355)regulation of DNA-templated transcription (GO:0006355)regulation of transcription by RNA polymerase II (GO:0006357)regulation of transcription by RNA polymerase II (GO:0006357)sequence-specific double-stranded DNA binding (GO:1990837)transcription regulator complex (GO:0005667)

Expression (TPM)

TFs regulating E2F4 0 TFs

Transcription factors with Perturb-seq knockdown data for E2F4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = E2F4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to E2F4

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of E2F4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr16:66,924,739–66,925,515	267.1 kb	Distal (>10kb) Multiome	395
chr16:66,933,957–66,934,849	257.7 kb	Distal (>10kb) Multiome	931
chr16:67,000,412–67,001,423	191.2 kb	Distal (>10kb) Multiome HiCAR	270
chr16:67,013,778–67,014,584	178.1 kb	Distal (>10kb) Multiome	594
chr16:67,017,916–67,018,379	174.1 kb	Distal (>10kb) Multiome	530
chr16:67,028,567–67,029,938	163.1 kb	Distal (>10kb) Multiome	1003
chr16:67,109,102–67,110,434	82.4 kb	Distal (>10kb) Multiome	895
chr16:67,149,282–67,151,406	41.5 kb	Distal (>10kb) Multiome	693
chr16:67,154,687–67,155,820	36.7 kb	Distal (>10kb) Multiome	593
chr16:67,160,628–67,161,206	31.1 kb	Distal (>10kb) Multiome	524
chr16:67,162,969–67,163,815	28.8 kb	Distal (>10kb) Multiome	238
chr16:67,169,105–67,171,287	22.6 kb	Distal (>10kb) Multiome	813
chr16:67,173,849–67,174,861	17.9 kb	Distal (>10kb) Multiome	809
chr16:67,177,863–67,178,664	13.9 kb	Distal (>10kb) Multiome	544
chr16:67,183,333–67,184,856	8.3 kb	Proximal (<10kb) Multiome	886
chr16:67,185,256–67,185,693	6.6 kb	Proximal (<10kb) Multiome	740
chr16:67,191,949–67,192,449	38 bp	At TSS Multiome	781
chr16:67,198,366–67,198,758	6.2 kb	Proximal (<10kb)	193
chr16:67,198,940–67,200,211	7.5 kb	Proximal (<10kb) Multiome	325
chr16:67,226,487–67,227,575	34.8 kb	Distal (>10kb) Multiome	909
chr16:67,236,439–67,237,267	44.6 kb	Distal (>10kb) Multiome	195
chr16:67,245,855–67,247,890	55.4 kb	Distal (>10kb) Multiome	1077
chr16:67,248,206–67,249,338	56.9 kb	Distal (>10kb) Multiome	467
chr16:67,262,177–67,262,869	70.3 kb	Distal (>10kb) Multiome	24
chr16:67,278,724–67,279,792	87.2 kb	Distal (>10kb) Multiome	869
chr16:67,326,413–67,327,343	134.7 kb	Distal (>10kb) Multiome	585
chr16:67,393,199–67,393,789	201.3 kb	Distal (>10kb) Multiome	377
chr16:67,396,006–67,396,718	204.3 kb	Distal (>10kb) Multiome	375
chr16:67,399,230–67,399,872	207.4 kb	Distal (>10kb) Multiome	211
chr16:67,415,996–67,416,812	224.2 kb	Distal (>10kb) Multiome	687
chr16:67,428,012–67,428,783	236.1 kb	Distal (>10kb) Multiome	382
chr16:67,430,407–67,432,610	238.9 kb	Distal (>10kb) Multiome	790
chr16:67,480,508–67,481,841	289.1 kb	Distal (>10kb) Multiome	1002

Genome Browser

Genomic view of the E2F4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.