Diphthamide is a post-translationally modified histidine residue present in elongation factor 2, and is the target of diphtheria toxin. This gene encodes a protein that contains a WD-40 domain, and is thought to be involved in diphthamide biosynthesis. A similar protein in yeast functions as a methylesterase, converting methylated diphthine to diphthine, which can then undergo amidation to produce diphthamide. [provided by RefSeq, Oct 2016]
Transcription factors with Perturb-seq knockdown data for DPH7. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DPH7 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DPH7, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:137,280,593–137,281,861 | 297.7 kb | Distal (>10kb) Multiome | 223 | |
| chr9:137,294,859–137,296,916 | 283.4 kb | Distal (>10kb) Multiome | 501 | |
| chr9:137,300,591–137,303,141 | 276.4 kb | Distal (>10kb) Multiome | 790 | |
| chr9:137,316,104–137,316,916 | 262.6 kb | Distal (>10kb) Multiome | 395 | |
| chr9:137,406,843–137,407,556 | 171.7 kb | Distal (>10kb) Multiome | 427 | |
| chr9:137,422,664–137,424,024 | 155.6 kb | Distal (>10kb) Multiome | 896 | |
| chr9:137,441,270–137,441,970 | 137.3 kb | Distal (>10kb) Multiome | 568 | |
| chr9:137,453,153–137,455,543 | 124.8 kb | Distal (>10kb) Multiome | 727 | |
| chr9:137,458,468–137,459,887 | 119.5 kb | Distal (>10kb) Multiome | 613 | |
| chr9:137,461,555–137,462,160 | 117.0 kb | Distal (>10kb) Multiome | 284 | |
| chr9:137,551,136–137,552,512 | 27.1 kb | Distal (>10kb) Multiome | 756 | |
| chr9:137,578,523–137,579,553 | 51 bp | At TSS Multiome | 910 | |
| chr9:137,589,526–137,591,323 | 11.7 kb | Distal (>10kb) Multiome | 623 | |
| chr9:137,604,440–137,607,003 | 26.5 kb | Distal (>10kb) Multiome | 675 | |
| chr9:137,618,064–137,619,880 | 40.1 kb | Distal (>10kb) Multiome | 867 | |
| chr9:137,723,975–137,724,439 | 145.3 kb | Distal (>10kb) Multiome | 115 | |
| chr9:137,732,255–137,733,025 | 153.8 kb | Distal (>10kb) Multiome | 20 | |
| chr9:137,851,599–137,852,229 | 273.0 kb | Distal (>10kb) Multiome | 104 | |
| chr9:137,876,903–137,879,307 | 298.7 kb | Distal (>10kb) Multiome | 452 |
Genomic view of the DPH7 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.