DCTN1

dynactin subunit 1

This gene encodes the largest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. Dynactin is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit interacts with dynein intermediate chain by its domains directly binding to dynein and binds to microtubules via a highly conserved glycine-rich cytoskeleton-associated protein (CAP-Gly) domain in its N-terminus. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause distal hereditary motor neuronopathy type VIIB (HMN7B) which is also known as distal spinal and bulbar muscular atrophy (dSBMA). [provided by RefSeq, Oct 2008]

Member of: DE-4 Developmental clusters: GC2

Biological processes 74 terms

axon (GO:0030424)axonal transport (GO:0098930)cell cortex (GO:0005938)cell cortex (GO:0005938)cell cortex (GO:0005938)cell cortex region (GO:0099738)cell leading edge (GO:0031252)centriolar subdistal appendage (GO:0120103)centriole (GO:0005814)centriole (GO:0005814)centriole-centriole cohesion (GO:0010457)centrosome (GO:0005813)centrosome (GO:0005813)cholesterol homeostasis (GO:0042632)ciliary basal body (GO:0036064)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoskeleton (GO:0005856)cytosol (GO:0005829)endosome membrane (GO:0010008)establishment of mitotic spindle orientation (GO:0000132)establishment of mitotic spindle orientation (GO:0000132)intercellular bridge (GO:0045171)kinetochore (GO:0000776)kinetochore (GO:0000776)lysosome to ER cholesterol transport (GO:0090120)maintenance of synapse structure (GO:0099558)membrane (GO:0016020)microtubule (GO:0005874)microtubule anchoring at centrosome (GO:0034454)microtubule associated complex (GO:0005875)microtubule associated complex (GO:0005875)microtubule binding (GO:0008017)microtubule binding (GO:0008017)microtubule binding (GO:0008017)microtubule cytoskeleton (GO:0015630)microtubule cytoskeleton (GO:0015630)microtubule plus-end (GO:0035371)microtubule plus-end (GO:0035371)mitotic cell cycle (GO:0000278)mitotic nuclear membrane disassembly (GO:0007077)motor behavior (GO:0061744)nervous system development (GO:0007399)neuromuscular junction development (GO:0007528)neuromuscular process (GO:0050905)neuron cellular homeostasis (GO:0070050)neuron projection (GO:0043005)neuron projection maintenance (GO:1990535)neuronal cell body (GO:0043025)non-motile cilium assembly (GO:1905515)nuclear envelope (GO:0005635)nuclear envelope (GO:0005635)nuclear migration (GO:0007097)positive regulation of microtubule nucleation (GO:0090063)positive regulation of microtubule polymerization (GO:0031116)positive regulation of microtubule polymerization (GO:0031116)positive regulation of neuromuscular junction development (GO:1904398)protein binding (GO:0005515)protein kinase binding (GO:0019901)protein-containing complex (GO:0032991)protein-containing complex (GO:0032991)regulation of mitotic spindle organization (GO:0060236)retrograde transport, endosome to Golgi (GO:0042147)retromer complex (GO:0030904)spindle (GO:0005819)spindle (GO:0005819)spindle pole (GO:0000922)spindle pole (GO:0000922)spindle pole (GO:0000922)sterol sensor activity (GO:0032935)tau protein binding (GO:0048156)tubulin binding (GO:0015631)ventral spinal cord development (GO:0021517)

Expression (TPM)

TFs regulating DCTN1 0 TFs

Transcription factors with Perturb-seq knockdown data for DCTN1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = DCTN1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to DCTN1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of DCTN1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr2:74,119,987–74,120,643	271.5 kb	Distal (>10kb) Multiome	969
chr2:74,147,573–74,148,843	243.8 kb	Distal (>10kb) Multiome	884
chr2:74,178,147–74,179,324	212.9 kb	Distal (>10kb) Multiome	869
chr2:74,187,993–74,188,519	203.6 kb	Distal (>10kb) Multiome	192
chr2:74,198,074–74,199,486	193.3 kb	Distal (>10kb) Multiome	1098
chr2:74,261,293–74,261,813	130.2 kb	Distal (>10kb) Multiome	184
chr2:74,374,253–74,375,246	17.1 kb	Distal (>10kb) Multiome	158
chr2:74,379,653–74,380,438	11.8 kb	Distal (>10kb) Multiome	229
chr2:74,391,129–74,392,484	96 bp	At TSS Multiome	876
chr2:74,421,273–74,422,464	29.9 kb	Distal (>10kb) Multiome	756
chr2:74,426,172–74,426,842	34.7 kb	Distal (>10kb) Multiome	88
chr2:74,440,246–74,442,723	50.3 kb	Distal (>10kb) Multiome	1050
chr2:74,454,612–74,455,413	63.2 kb	Distal (>10kb) Multiome	982
chr2:74,457,177–74,459,169	66.4 kb	Distal (>10kb) Multiome	934
chr2:74,464,912–74,465,776	73.5 kb	Distal (>10kb) Multiome	842
chr2:74,472,106–74,473,059	80.8 kb	Distal (>10kb) Multiome	1071
chr2:74,481,955–74,483,673	91.3 kb	Distal (>10kb) Multiome	1031
chr2:74,497,707–74,500,027	107.6 kb	Distal (>10kb) Multiome	553
chr2:74,502,724–74,504,160	111.4 kb	Distal (>10kb) Multiome	816
chr2:74,506,905–74,508,524	115.8 kb	Distal (>10kb) Multiome	1063
chr2:74,515,477–74,516,725	124.6 kb	Distal (>10kb) Multiome	262
chr2:74,529,210–74,530,815	138.0 kb	Distal (>10kb) Multiome	988
chr2:74,548,706–74,549,795	157.6 kb	Distal (>10kb) Multiome	625
chr2:74,553,035–74,555,636	162.7 kb	Distal (>10kb) Multiome	768
chr2:74,647,804–74,648,489	256.4 kb	Distal (>10kb) Multiome	232
chr2:74,653,808–74,654,908	262.4 kb	Distal (>10kb) Multiome	658

Genome Browser

Genomic view of the DCTN1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.