This gene encodes a flavin adenine dinucleotide-binding protein that is a key component of the circadian core oscillator complex, which regulates the circadian clock. This gene is upregulated by CLOCK/ARNTL heterodimers but then represses this upregulation in a feedback loop using PER/CRY heterodimers to interact with CLOCK/ARNTL. Polymorphisms in this gene have been associated with altered sleep patterns. The encoded protein is widely conserved across plants and animals. Loss of the related gene in mouse results in a shortened circadian cycle in complete darkness. [provided by RefSeq, Jan 2014]
Transcription factors with Perturb-seq knockdown data for CRY1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CRY1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CRY1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr12:106,903,307–106,903,916 | 189.8 kb | Distal (>10kb) Multiome | 193 | |
| chr12:106,954,854–106,956,979 | 137.8 kb | Distal (>10kb) Multiome | 927 | |
| chr12:106,986,640–106,987,674 | 106.4 kb | Distal (>10kb) Multiome | 764 | |
| chr12:107,092,012–107,092,634 | 914 bp | At TSS | 402 | |
| chr12:107,092,776–107,094,398 | 267 bp | At TSS Multiome | 884 | |
| chr12:107,122,282–107,122,891 | 29.1 kb | Distal (>10kb) Multiome | 88 | |
| chr12:107,317,739–107,321,052 | 224.8 kb | Distal (>10kb) Multiome | 752 |
Genomic view of the CRY1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.