Involved in negative regulation of barbed-end actin filament capping. Predicted to be located in cytosol. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for CRACD. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CRACD upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CRACD, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:55,853,417–55,854,168 | 195.4 kb | Distal (>10kb) Multiome | 698 | |
| chr4:55,948,442–55,949,600 | 100.2 kb | Distal (>10kb) Multiome | 993 | |
| chr4:56,048,601–56,050,047 | 715 bp | At TSS Multiome | 655 | |
| chr4:56,055,093–56,055,833 | 6.3 kb | Proximal (<10kb) Multiome | 115 | |
| chr4:56,323,870–56,324,663 | 275.3 kb | Distal (>10kb) Multiome HiCAR | 70 |
Genomic view of the CRACD locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.