The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CPT1A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CPT1A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CPT1A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:68,683,947–68,685,063 | 155.1 kb | Distal (>10kb) Multiome | 317 | |
| chr11:68,749,819–68,752,106 | 88.2 kb | Distal (>10kb) Multiome | 601 | |
| chr11:68,839,165–68,839,925 | 272 bp | At TSS Multiome | 821 | |
| chr11:68,840,765–68,842,245 | 2.2 kb | Proximal (<10kb) Multiome | 567 | |
| chr11:68,843,712–68,844,382 | 4.4 kb | Proximal (<10kb) Multiome | 675 | |
| chr11:68,855,267–68,855,945 | 15.9 kb | Distal (>10kb) Multiome | 230 | |
| chr11:68,879,344–68,879,843 | 39.9 kb | Distal (>10kb) Multiome | 69 | |
| chr11:68,903,762–68,904,282 | 64.2 kb | Distal (>10kb) Multiome | 1004 | |
| chr11:69,010,998–69,011,634 | 171.5 kb | Distal (>10kb) Multiome | 255 | |
| chr11:69,013,121–69,013,629 | 173.8 kb | Distal (>10kb) Multiome | 143 | |
| chr11:69,048,479–69,049,470 | 209.2 kb | Distal (>10kb) Multiome | 583 | |
| chr11:69,122,768–69,123,396 | 283.3 kb | Distal (>10kb) Multiome | 565 | |
| chr11:69,674,751–69,675,727 | 835.7 kb | Distal (>10kb) Multiome HiCAR | 862 |
Genomic view of the CPT1A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.