The product of this gene is an essential upstream regulator of checkpoint kinase 1 and triggers a checkpoint arrest of the cell cycle in response to replicative stress or DNA damage. The protein is also required for efficient DNA replication during a normal S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
Transcription factors with Perturb-seq knockdown data for CLSPN. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CLSPN upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CLSPN, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:35,533,667–35,534,085 | 236.1 kb | Distal (>10kb) Multiome | 64 | |
| chr1:35,556,848–35,558,023 | 212.4 kb | Distal (>10kb) Multiome | 894 | |
| chr1:35,573,081–35,573,635 | 196.6 kb | Distal (>10kb) Multiome HiCAR | 174 | |
| chr1:35,577,045–35,577,930 | 192.3 kb | Distal (>10kb) Multiome HiCAR | 235 | |
| chr1:35,641,114–35,641,872 | 128.4 kb | Distal (>10kb) Multiome | 824 | |
| chr1:35,707,208–35,708,693 | 62.1 kb | Distal (>10kb) Multiome | 388 | |
| chr1:35,717,979–35,719,281 | 51.1 kb | Distal (>10kb) Multiome | 558 | |
| chr1:35,769,208–35,770,406 | 7 bp | At TSS Multiome | 929 | |
| chr1:35,807,537–35,808,824 | 38.1 kb | Distal (>10kb) Multiome | 575 | |
| chr1:35,882,591–35,884,430 | 113.1 kb | Distal (>10kb) Multiome | 802 | |
| chr1:35,885,650–35,886,507 | 116.2 kb | Distal (>10kb) Multiome | 156 | |
| chr1:35,930,610–35,931,886 | 161.0 kb | Distal (>10kb) Multiome | 903 |
Genomic view of the CLSPN locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.