Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CILK1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CILK1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CILK1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:52,413,171–52,413,920 | 648.2 kb | Distal (>10kb) Multiome HiCAR | 46 | |
| chr6:52,419,778–52,421,057 | 641.5 kb | Distal (>10kb) Multiome HiCAR | 913 | |
| chr6:52,855,940–52,856,582 | 205.5 kb | Distal (>10kb) Multiome | 166 | |
| chr6:52,861,065–52,861,720 | 200.3 kb | Distal (>10kb) Multiome | 105 | |
| chr6:52,994,589–52,996,420 | 66.3 kb | Distal (>10kb) Multiome HiCAR | 1101 | |
| chr6:53,060,233–53,060,576 | 1.2 kb | Proximal (<10kb) | 31 | |
| chr6:53,061,219–53,062,138 | 70 bp | At TSS Multiome | 961 | |
| chr6:53,064,550–53,066,422 | 3.3 kb | Proximal (<10kb) Multiome | 934 | |
| chr6:53,347,548–53,349,468 | 286.4 kb | Distal (>10kb) Multiome | 976 | |
| chr6:53,358,501–53,360,085 | 297.7 kb | Distal (>10kb) Multiome | 1019 |
Genomic view of the CILK1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.