This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CHUK. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHUK upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHUK, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr10:100,009,381–100,010,564 | 219.7 kb | Distal (>10kb) Multiome | 647 | |
| chr10:100,185,381–100,186,650 | 43.5 kb | Distal (>10kb) Multiome | 913 | |
| chr10:100,228,957–100,229,948 | 17 bp | At TSS Multiome | 759 | |
| chr10:100,267,386–100,267,986 | 38.1 kb | Distal (>10kb) Multiome | 715 | |
| chr10:100,286,018–100,287,021 | 57.0 kb | Distal (>10kb) Multiome | 849 | |
| chr10:100,346,401–100,347,640 | 117.3 kb | Distal (>10kb) Multiome | 919 | |
| chr10:100,359,067–100,359,724 | 129.7 kb | Distal (>10kb) Multiome | 425 | |
| chr10:100,372,854–100,374,386 | 143.9 kb | Distal (>10kb) Multiome | 964 | |
| chr10:100,481,576–100,482,049 | 252.1 kb | Distal (>10kb) Multiome | 298 | |
| chr10:100,482,268–100,483,078 | 253.2 kb | Distal (>10kb) Multiome | 497 | |
| chr10:100,485,778–100,486,586 | 256.5 kb | Distal (>10kb) Multiome | 161 | |
| chr10:100,518,832–100,519,948 | 289.8 kb | Distal (>10kb) Multiome | 417 | |
| chr10:100,529,444–100,530,236 | 300.3 kb | Distal (>10kb) Multiome | 619 |
Genomic view of the CHUK locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.