CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for CHMP5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CHMP5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CHMP5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr9:33,000,845–33,002,021 | 263.5 kb | Distal (>10kb) Multiome | 802 | |
| chr9:33,024,523–33,026,243 | 239.8 kb | Distal (>10kb) Multiome | 972 | |
| chr9:33,044,108–33,044,981 | 220.5 kb | Distal (>10kb) Multiome | 451 | |
| chr9:33,076,221–33,076,965 | 188.3 kb | Distal (>10kb) Multiome | 804 | |
| chr9:33,166,320–33,168,338 | 97.7 kb | Distal (>10kb) Multiome | 849 | |
| chr9:33,171,750–33,173,178 | 92.5 kb | Distal (>10kb) Multiome | 359 | |
| chr9:33,264,068–33,265,475 | 252 bp | At TSS Multiome | 790 | |
| chr9:33,267,199–33,267,770 | 2.4 kb | Proximal (<10kb) Multiome | 106 | |
| chr9:33,290,056–33,291,152 | 25.4 kb | Distal (>10kb) Multiome | 1075 | |
| chr9:33,381,203–33,381,714 | 116.5 kb | Distal (>10kb) Multiome | 134 | |
| chr9:33,415,341–33,415,979 | 150.6 kb | Distal (>10kb) Multiome | 774 | |
| chr9:33,447,056–33,448,058 | 182.6 kb | Distal (>10kb) Multiome | 547 | |
| chr9:33,452,742–33,453,295 | 188.0 kb | Distal (>10kb) Multiome | 374 | |
| chr9:33,473,593–33,474,250 | 208.9 kb | Distal (>10kb) Multiome | 635 | |
| chr9:33,515,277–33,515,972 | 250.5 kb | Distal (>10kb) Multiome | 49 | |
| chr9:33,523,393–33,524,316 | 258.7 kb | Distal (>10kb) Multiome | 303 |
Genomic view of the CHMP5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.