CFL1

cofilin 1 | CFL

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Member of: DE-1 DE-1.35

Biological processes 92 terms

Rho protein signal transduction (GO:0007266)actin binding (GO:0003779)actin cytoskeleton (GO:0015629)actin cytoskeleton (GO:0015629)actin cytoskeleton organization (GO:0030036)actin filament binding (GO:0051015)actin filament binding (GO:0051015)actin filament binding (GO:0051015)actin filament binding (GO:0051015)actin filament depolymerization (GO:0030042)actin filament depolymerization (GO:0030042)actin filament depolymerization (GO:0030042)actin filament fragmentation (GO:0030043)actin filament severing (GO:0051014)actin filament severing activity (GO:0003789)actin polymerization or depolymerization (GO:0008154)axon (GO:0030424)cell leading edge (GO:0031252)cell-cell junction (GO:0005911)cellular response to epidermal growth factor stimulus (GO:0071364)cellular response to ether (GO:0071362)cellular response to hydrogen peroxide (GO:0070301)cellular response to insulin-like growth factor stimulus (GO:1990314)cellular response to interleukin-1 (GO:0071347)cellular response to interleukin-6 (GO:0071354)cellular response to tumor necrosis factor (GO:0071356)cofilin-actin rod (GO:0090732)cortical actin cytoskeleton (GO:0030864)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoplasm (GO:0005737)cytoskeleton (GO:0005856)cytosol (GO:0005829)dendritic spine (GO:0043197)establishment of spindle localization (GO:0051293)establishment of spindle localization (GO:0051293)extracellular exosome (GO:0070062)extracellular region (GO:0005576)filopodium (GO:0030175)focal adhesion (GO:0005925)focal adhesion (GO:0005925)glutamatergic synapse (GO:0098978)growth cone (GO:0030426)hippocampus development (GO:0021766)host-mediated activation of viral process (GO:0044794)lamellipodium (GO:0030027)lamellipodium (GO:0030027)lamellipodium (GO:0030027)lamellipodium membrane (GO:0031258)membrane (GO:0016020)mitochondrial membrane (GO:0031966)mitotic cytokinesis (GO:0000281)modification of postsynaptic actin cytoskeleton (GO:0098885)modulation of chemical synaptic transmission (GO:0050804)negative regulation of actin filament bundle assembly (GO:0032232)negative regulation of actin filament depolymerization (GO:0030835)negative regulation of apoptotic process (GO:0043066)negative regulation of cell adhesion (GO:0007162)negative regulation of cell motility (GO:2000146)negative regulation of dendritic spine maintenance (GO:1902951)negative regulation of lamellipodium assembly (GO:0010593)negative regulation of postsynaptic density organization (GO:1905875)negative regulation of unidimensional cell growth (GO:0051511)neuronal cell body (GO:0043025)nuclear matrix (GO:0016363)nucleus (GO:0005634)phosphatidylinositol bisphosphate binding (GO:1902936)positive regulation of actin filament depolymerization (GO:0030836)positive regulation of barbed-end actin filament capping (GO:2000814)positive regulation of cell growth (GO:0030307)positive regulation of cell motility (GO:2000147)positive regulation of dendritic spine development (GO:0060999)positive regulation of embryonic development (GO:0040019)positive regulation of embryonic development (GO:0040019)positive regulation of establishment of cell polarity regulating cell shape (GO:2000784)positive regulation of focal adhesion assembly (GO:0051894)positive regulation of lamellipodium assembly (GO:0010592)positive regulation of protein localization to cell leading edge (GO:1905873)positive regulation of proteolysis (GO:0045862)positive regulation of synaptic plasticity (GO:0031915)postsynaptic density, intracellular component (GO:0099092)protein binding (GO:0005515)protein import into nucleus (GO:0006606)protein phosphatase binding (GO:0019903)regulation of dendritic spine morphogenesis (GO:0061001)response to activity (GO:0014823)response to virus (GO:0009615)ruffle membrane (GO:0032587)signaling receptor binding (GO:0005102)synaptic membrane (GO:0097060)vesicle (GO:0031982)

Expression (TPM)

TFs regulating CFL1 0 TFs

Transcription factors with Perturb-seq knockdown data for CFL1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CFL1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to CFL1

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CFL1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr11:65,569,718–65,570,745	287.9 kb	Distal (>10kb) Multiome	930
chr11:65,571,973–65,573,008	285.7 kb	Distal (>10kb) Multiome	420
chr11:65,573,788–65,575,269	283.9 kb	Distal (>10kb) Multiome	742
chr11:65,575,601–65,576,322	282.2 kb	Distal (>10kb) Multiome	527
chr11:65,591,808–65,592,698	266.0 kb	Distal (>10kb) Multiome	169
chr11:65,606,536–65,607,917	251.0 kb	Distal (>10kb) Multiome	376
chr11:65,613,478–65,614,645	244.0 kb	Distal (>10kb) Multiome	702
chr11:65,614,954–65,616,793	242.6 kb	Distal (>10kb) Multiome	830
chr11:65,637,768–65,638,920	220.1 kb	Distal (>10kb) Multiome	740
chr11:65,641,918–65,642,633	216.0 kb	Distal (>10kb) Multiome	155
chr11:65,646,814–65,647,818	210.9 kb	Distal (>10kb) Multiome	270
chr11:65,652,106–65,653,317	205.4 kb	Distal (>10kb) Multiome	901
chr11:65,662,517–65,663,930	195.1 kb	Distal (>10kb) Multiome	839
chr11:65,711,437–65,712,942	145.9 kb	Distal (>10kb) Multiome HiCAR	1143
chr11:65,720,215–65,720,955	137.5 kb	Distal (>10kb) Multiome	821
chr11:65,779,808–65,781,988	78.1 kb	Distal (>10kb) Multiome	764
chr11:65,785,898–65,789,101	69.8 kb	Distal (>10kb) Multiome	478
chr11:65,817,968–65,818,502	39.9 kb	Distal (>10kb) Multiome	694
chr11:65,833,325–65,834,189	24.4 kb	Distal (>10kb) Multiome	396
chr11:65,856,804–65,858,748	1.1 kb	Proximal (<10kb) Multiome	972
chr11:65,859,034–65,861,240	2.4 kb	Proximal (<10kb) Multiome	1012
chr11:65,871,920–65,874,072	15.5 kb	Distal (>10kb) Multiome	741
chr11:65,887,987–65,889,162	30.4 kb	Distal (>10kb) Multiome	824
chr11:65,890,253–65,891,457	32.5 kb	Distal (>10kb) Multiome	895
chr11:65,899,867–65,900,779	42.4 kb	Distal (>10kb) Multiome	740
chr11:65,918,507–65,920,266	61.1 kb	Distal (>10kb) Multiome	992
chr11:65,961,271–65,962,172	103.5 kb	Distal (>10kb) Multiome	834
chr11:66,001,882–66,002,962	144.2 kb	Distal (>10kb) Multiome	1022
chr11:66,007,227–66,007,703	149.3 kb	Distal (>10kb) Multiome	100
chr11:66,022,341–66,022,903	164.4 kb	Distal (>10kb) Multiome	466
chr11:66,045,170–66,045,637	187.2 kb	Distal (>10kb) Multiome	394
chr11:66,048,912–66,049,460	191.0 kb	Distal (>10kb) Multiome	83
chr11:66,051,716–66,052,760	194.0 kb	Distal (>10kb) Multiome	901
chr11:66,069,690–66,071,102	212.0 kb	Distal (>10kb) Multiome	622

Genome Browser

Genomic view of the CFL1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.