The protein encoded by this gene is an inner protein of the kinetochore, the multi-protein complex that binds spindle microtubules to regulate chromosome segregation during cell division. It belongs to the constitutive centromere-associated network protein group, whose members interact with outer kinetochore proteins and help to maintain centromere identity at each cell division cycle. The protein is structurally related to GTPases but cannot bind guanosine triphosphate. A point mutation that affects interaction with another constitutive centromere-associated network protein, CENP-I, impairs kinetochore assembly and chromosome alignment, suggesting that it is required for kinetochore formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]
Transcription factors with Perturb-seq knockdown data for CENPM. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPM upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPM, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:41,666,496–41,667,405 | 280.3 kb | Distal (>10kb) Multiome HiCAR | 725 | |
| chr22:41,697,169–41,697,792 | 249.8 kb | Distal (>10kb) Multiome HiCAR | 464 | |
| chr22:41,800,274–41,801,345 | 146.5 kb | Distal (>10kb) Multiome | 816 | |
| chr22:41,832,291–41,834,215 | 114.2 kb | Distal (>10kb) Multiome | 1023 | |
| chr22:41,845,927–41,846,489 | 101.0 kb | Distal (>10kb) Multiome | 271 | |
| chr22:41,909,821–41,910,788 | 36.9 kb | Distal (>10kb) Multiome | 251 | |
| chr22:41,913,915–41,914,497 | 33.0 kb | Distal (>10kb) Multiome | 143 | |
| chr22:41,914,624–41,915,454 | 32.1 kb | Distal (>10kb) Multiome | 273 | |
| chr22:41,919,501–41,920,436 | 27.2 kb | Distal (>10kb) Multiome | 337 | |
| chr22:41,926,076–41,926,820 | 20.9 kb | Distal (>10kb) Multiome | 442 | |
| chr22:41,941,343–41,941,562 | 5.1 kb | Proximal (<10kb) | 342 | |
| chr22:41,945,821–41,947,304 | 213 bp | At TSS Multiome | 896 | |
| chr22:41,957,553–41,958,332 | 10.7 kb | Distal (>10kb) Multiome | 456 | |
| chr22:41,976,503–41,977,084 | 29.6 kb | Distal (>10kb) Multiome | 294 | |
| chr22:42,070,303–42,071,253 | 123.7 kb | Distal (>10kb) Multiome | 833 | |
| chr22:42,074,171–42,074,703 | 127.3 kb | Distal (>10kb) Multiome | 178 | |
| chr22:42,079,194–42,080,293 | 132.6 kb | Distal (>10kb) Multiome | 872 | |
| chr22:42,090,032–42,091,609 | 143.8 kb | Distal (>10kb) Multiome | 910 | |
| chr22:42,444,134–42,444,723 | 497.3 kb | Distal (>10kb) Multiome HiCAR | 574 |
Genomic view of the CENPM locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.