This gene encodes a centromere protein that is a component of the CENPA-NAC (nucleosome-associated) complex. This complex is critical for accurate chromosome alignment and segregation and it ensures proper mitotic progression. This protein regulates the recruitment of kinetochore-associated proteins that are required to generate the spindle checkpoint signal. The product of this gene is involved in the response of gonadal tissues to follicle-stimulating hormone. Mutations in this gene may be involved in human X-linked disorders of gonadal development and gametogenesis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 13. [provided by RefSeq, Jan 2016]
Transcription factors with Perturb-seq knockdown data for CENPI. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPI upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPI, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chrX:100,819,952–100,820,670 | 277.9 kb | Distal (>10kb) Multiome | 553 | |
| chrX:100,928,599–100,929,634 | 169.1 kb | Distal (>10kb) Multiome | 309 | |
| chrX:101,051,311–101,052,275 | 46.3 kb | Distal (>10kb) Multiome | 546 | |
| chrX:101,097,632–101,098,463 | 148 bp | At TSS Multiome | 508 | |
| chrX:101,348,449–101,349,149 | 250.6 kb | Distal (>10kb) Multiome | 567 | |
| chrX:101,390,531–101,391,386 | 292.8 kb | Distal (>10kb) Multiome | 762 |
Genomic view of the CENPI locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.