Centromere and kinetochore proteins play a critical role in centromere structure, kinetochore formation, and sister chromatid separation. The protein encoded by this gene colocalizes with inner kinetochore plate proteins CENP-A and CENP-C in both interphase and metaphase. It localizes outside of centromeric heterochromatin, where CENP-B is localized, and inside the kinetochore corona, where CENP-E is localized during prometaphase. It is thought that this protein can bind to itself, as well as to CENP-A, CENP-B or CENP-C. Multimers of the protein localize constitutively to the inner kinetochore plate and play an important role in the organization and function of the active centromere-kinetochore complex. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CENPH. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPH upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPH, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:68,922,292–68,922,890 | 267.0 kb | Distal (>10kb) Multiome | 63 | |
| chr5:69,093,377–69,094,772 | 95.6 kb | Distal (>10kb) Multiome | 952 | |
| chr5:69,166,575–69,167,819 | 22.5 kb | Distal (>10kb) Multiome | 840 | |
| chr5:69,188,979–69,190,115 | 21 bp | At TSS Multiome | 898 | |
| chr5:69,217,542–69,218,587 | 28.4 kb | Distal (>10kb) Multiome | 898 | |
| chr5:69,234,302–69,235,305 | 45.3 kb | Distal (>10kb) Multiome | 732 | |
| chr5:69,300,544–69,301,286 | 111.3 kb | Distal (>10kb) Multiome | 217 | |
| chr5:69,368,998–69,370,235 | 180.1 kb | Distal (>10kb) Multiome | 956 | |
| chr5:69,414,852–69,415,897 | 225.6 kb | Distal (>10kb) Multiome | 714 |
Genomic view of the CENPH locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.