This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CENPF. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPF upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPF, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:214,316,395–214,317,010 | 286.4 kb | Distal (>10kb) Multiome HiCAR | 226 | |
| chr1:214,317,130–214,317,628 | 285.7 kb | Distal (>10kb) Multiome HiCAR | 145 | |
| chr1:214,378,748–214,380,041 | 223.6 kb | Distal (>10kb) Multiome HiCAR | 127 | |
| chr1:214,446,675–214,447,519 | 156.1 kb | Distal (>10kb) Multiome | 415 | |
| chr1:214,550,016–214,553,159 | 51.4 kb | Distal (>10kb) Multiome | 869 | |
| chr1:214,602,774–214,603,730 | 38 bp | At TSS Multiome | 887 | |
| chr1:214,603,866–214,604,264 | 682 bp | At TSS | 156 | |
| chr1:214,683,504–214,684,332 | 80.7 kb | Distal (>10kb) Multiome | 116 | |
| chr1:215,566,760–215,568,510 | 964.3 kb | Distal (>10kb) Multiome HiCAR | 1008 |
Genomic view of the CENPF locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.