Centrosome-associated protein E (CENPE) is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. This protein is required for stable spindle microtubule capture at kinetochores which is a necessary step in chromosome alignment during prometaphase. This protein also couples chromosome position to microtubule depolymerizing activity. Alternative splicing results in multiple transcript variants encoding distinct protein isoforms. [provided by RefSeq, Nov 2014]
Transcription factors with Perturb-seq knockdown data for CENPE. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CENPE upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CENPE, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr4:103,018,881–103,020,188 | 178.7 kb | Distal (>10kb) Multiome | 882 | |
| chr4:103,075,865–103,077,254 | 121.8 kb | Distal (>10kb) Multiome | 845 | |
| chr4:103,197,655–103,198,939 | 51 bp | At TSS Multiome | 812 | |
| chr4:103,207,594–103,208,167 | 9.2 kb | Proximal (<10kb) | 143 | |
| chr4:103,429,192–103,430,032 | 231.2 kb | Distal (>10kb) Multiome | 259 | |
| chr4:103,477,555–103,478,976 | 280.0 kb | Distal (>10kb) Multiome | 202 | |
| chr4:103,946,181–103,949,124 | 750.4 kb | Distal (>10kb) Multiome HiCAR | 323 |
Genomic view of the CENPE locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.