The chaperonin containing TCP1 (MIM 186980) complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin (see MIM 102560) and tubulin (see MIM 191130), as well as alpha-transducin (MIM 139330) (Won et al., 1998 [PubMed 9819444]).[supplied by OMIM, Mar 2008]
Transcription factors with Perturb-seq knockdown data for CCT4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CCT4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CCT4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr2:61,694,419–61,695,205 | 193.7 kb | Distal (>10kb) Multiome | 599 | |
| chr2:61,763,751–61,764,826 | 124.1 kb | Distal (>10kb) Multiome | 975 | |
| chr2:61,787,728–61,788,473 | 100.3 kb | Distal (>10kb) Multiome | 51 | |
| chr2:61,793,349–61,793,828 | 94.9 kb | Distal (>10kb) Multiome | 309 | |
| chr2:61,853,553–61,855,487 | 34.4 kb | Distal (>10kb) Multiome | 973 | |
| chr2:61,888,195–61,889,300 | 205 bp | At TSS Multiome | 1042 | |
| chr2:61,905,370–61,906,012 | 17.2 kb | Distal (>10kb) Multiome | 819 | |
| chr2:61,924,970–61,925,711 | 37.0 kb | Distal (>10kb) Multiome | 28 | |
| chr2:62,108,942–62,110,921 | 221.5 kb | Distal (>10kb) Multiome | 186 | |
| chr2:62,370,563–62,371,206 | 482.6 kb | Distal (>10kb) Multiome HiCAR | 41 |
Genomic view of the CCT4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.