This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family . The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The protein may play an important role in the epigenetic control of chromatin structure and gene expression. Several related pseudogenes are located on chromosomes 1, 3, and X. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CBX1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CBX1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CBX1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:47,831,256–47,831,955 | 269.8 kb | Distal (>10kb) Multiome | 856 | |
| chr17:47,840,790–47,841,702 | 260.1 kb | Distal (>10kb) Multiome | 833 | |
| chr17:47,847,253–47,848,429 | 253.7 kb | Distal (>10kb) Multiome | 193 | |
| chr17:47,850,617–47,851,614 | 250.2 kb | Distal (>10kb) Multiome | 694 | |
| chr17:47,895,361–47,896,955 | 205.2 kb | Distal (>10kb) Multiome | 1031 | |
| chr17:47,902,020–47,902,508 | 199.2 kb | Distal (>10kb) Multiome | 101 | |
| chr17:47,941,283–47,941,880 | 159.8 kb | Distal (>10kb) Multiome | 958 | |
| chr17:47,970,487–47,971,547 | 130.4 kb | Distal (>10kb) Multiome | 1061 | |
| chr17:48,023,351–48,024,470 | 77.8 kb | Distal (>10kb) Multiome | 591 | |
| chr17:48,024,599–48,025,777 | 76.1 kb | Distal (>10kb) Multiome | 759 | |
| chr17:48,026,284–48,026,933 | 74.7 kb | Distal (>10kb) Multiome | 543 | |
| chr17:48,047,576–48,049,198 | 53.1 kb | Distal (>10kb) Multiome | 1134 | |
| chr17:48,053,836–48,055,155 | 46.9 kb | Distal (>10kb) Multiome | 327 | |
| chr17:48,055,581–48,056,000 | 45.4 kb | Distal (>10kb) Multiome | 337 | |
| chr17:48,100,593–48,101,963 | 37 bp | At TSS Multiome | 974 | |
| chr17:48,107,198–48,108,007 | 6.2 kb | Proximal (<10kb) Multiome | 841 | |
| chr17:48,160,363–48,161,134 | 59.4 kb | Distal (>10kb) Multiome | 299 |
Genomic view of the CBX1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.