This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for CALD1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = CALD1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of CALD1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:134,547,422–134,548,048 | 232.0 kb | Distal (>10kb) Multiome | 442 | |
| chr7:134,646,328–134,647,406 | 132.9 kb | Distal (>10kb) Multiome | 748 | |
| chr7:134,779,110–134,780,363 | 68 bp | At TSS Multiome | 408 | |
| chr7:134,882,180–134,882,312 | 9.1 kb | Proximal (<10kb) | 5 | |
| chr7:134,882,592–134,882,799 | 8.7 kb | Proximal (<10kb) | 19 | |
| chr7:134,890,877–134,892,305 | 111.9 kb | Distal (>10kb) Multiome | 673 | |
| chr7:134,899,410–134,899,751 | 8.0 kb | Proximal (<10kb) | 170 | |
| chr7:134,986,110–134,987,248 | 206.9 kb | Distal (>10kb) Multiome | 1029 | |
| chr7:135,170,026–135,171,132 | 391.1 kb | Distal (>10kb) Multiome | 1132 |
Genomic view of the CALD1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.