Transcription factors with Perturb-seq knockdown data for C1orf21. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = C1orf21 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of C1orf21, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:184,156,878–184,157,773 | 229.7 kb | Distal (>10kb) Multiome | 202 | |
| chr1:184,190,240–184,191,549 | 196.0 kb | Distal (>10kb) Multiome | 214 | |
| chr1:184,242,238–184,242,897 | 144.4 kb | Distal (>10kb) Multiome | 250 | |
| chr1:184,244,558–184,245,746 | 141.8 kb | Distal (>10kb) Multiome | 42 | |
| chr1:184,313,571–184,314,257 | 73.2 kb | Distal (>10kb) Multiome | 94 | |
| chr1:184,350,363–184,350,931 | 36.3 kb | Distal (>10kb) Multiome | 97 | |
| chr1:184,379,425–184,379,603 | 7.4 kb | Proximal (<10kb) | 70 | |
| chr1:184,381,278–184,382,270 | 4.8 kb | Proximal (<10kb) | 168 | |
| chr1:184,386,204–184,388,132 | 119 bp | At TSS Multiome | 664 | |
| chr1:184,582,004–184,583,178 | 195.4 kb | Distal (>10kb) Multiome | 114 | |
| chr1:184,663,783–184,664,949 | 277.2 kb | Distal (>10kb) Multiome HiCAR | 369 | |
| chr1:184,695,922–184,696,742 | 309.4 kb | Distal (>10kb) Multiome HiCAR | 40 |
Genomic view of the C1orf21 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.