This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Aug 2020]
Transcription factors with Perturb-seq knockdown data for BID. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BID upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BID, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:17,084,375–17,085,889 | 689.6 kb | Distal (>10kb) Multiome HiCAR | 653 | |
| chr22:17,089,112–17,089,816 | 685.1 kb | Distal (>10kb) Multiome HiCAR | 16 | |
| chr22:17,119,575–17,121,998 | 653.0 kb | Distal (>10kb) Multiome HiCAR | 547 | |
| chr22:17,628,467–17,629,189 | 145.6 kb | Distal (>10kb) Multiome | 714 | |
| chr22:17,638,195–17,639,235 | 135.7 kb | Distal (>10kb) Multiome | 934 | |
| chr22:17,773,581–17,774,636 | 384 bp | At TSS Multiome | 486 | |
| chr22:17,921,809–17,922,254 | 147.7 kb | Distal (>10kb) Multiome | 99 | |
| chr22:17,960,960–17,961,542 | 186.8 kb | Distal (>10kb) Multiome | 44 | |
| chr22:18,000,452–18,002,000 | 227.1 kb | Distal (>10kb) Multiome | 878 | |
| chr22:18,023,594–18,024,038 | 249.2 kb | Distal (>10kb) Multiome | 438 |
Genomic view of the BID locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.