This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for BCLAF1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BCLAF1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BCLAF1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:135,497,226–135,498,795 | 792.1 kb | Distal (>10kb) Multiome HiCAR | 1084 | |
| chr6:136,249,441–136,251,347 | 39.5 kb | Distal (>10kb) Multiome | 921 | |
| chr6:136,288,756–136,290,871 | 32 bp | At TSS Multiome | 1158 | |
| chr6:136,499,147–136,499,983 | 209.7 kb | Distal (>10kb) Multiome | 207 | |
| chr6:136,526,121–136,527,142 | 236.8 kb | Distal (>10kb) Multiome | 235 | |
| chr6:136,549,356–136,551,131 | 260.7 kb | Distal (>10kb) Multiome | 722 |
Genomic view of the BCLAF1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.