The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]
Transcription factors with Perturb-seq knockdown data for BAG4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = BAG4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of BAG4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr8:37,898,759–37,900,024 | 277.3 kb | Distal (>10kb) Multiome | 956 | |
| chr8:37,915,980–37,916,519 | 260.5 kb | Distal (>10kb) Multiome | 465 | |
| chr8:37,965,216–37,966,371 | 211.2 kb | Distal (>10kb) Multiome HiCAR | 177 | |
| chr8:38,015,136–38,015,926 | 161.3 kb | Distal (>10kb) Multiome | 59 | |
| chr8:38,029,913–38,031,249 | 146.4 kb | Distal (>10kb) Multiome | 948 | |
| chr8:38,066,984–38,067,582 | 109.6 kb | Distal (>10kb) Multiome | 414 | |
| chr8:38,070,928–38,072,232 | 105.1 kb | Distal (>10kb) Multiome | 282 | |
| chr8:38,104,797–38,106,255 | 71.2 kb | Distal (>10kb) Multiome | 954 | |
| chr8:38,108,727–38,109,662 | 67.8 kb | Distal (>10kb) Multiome | 83 | |
| chr8:38,175,948–38,177,501 | 123 bp | At TSS Multiome | 870 | |
| chr8:38,184,514–38,184,659 | 7.9 kb | Proximal (<10kb) | 212 | |
| chr8:38,230,935–38,232,445 | 54.7 kb | Distal (>10kb) Multiome | 904 | |
| chr8:38,268,159–38,269,673 | 92.4 kb | Distal (>10kb) Multiome | 795 | |
| chr8:38,381,367–38,383,496 | 205.5 kb | Distal (>10kb) Multiome | 956 | |
| chr8:38,385,791–38,387,334 | 209.6 kb | Distal (>10kb) Multiome | 816 | |
| chr8:38,440,285–38,442,102 | 264.0 kb | Distal (>10kb) Multiome | 293 | |
| chr8:38,442,374–38,442,980 | 265.8 kb | Distal (>10kb) Multiome | 254 | |
| chr8:38,467,549–38,469,321 | 291.8 kb | Distal (>10kb) Multiome | 775 | |
| chr8:38,471,859–38,472,443 | 295.3 kb | Distal (>10kb) Multiome | 84 | |
| chr8:38,734,447–38,735,152 | 557.9 kb | Distal (>10kb) Multiome HiCAR | 738 |
Genomic view of the BAG4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.