This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes in human. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. This gene produces multiple protein isoforms - some of which are predicted to lack the N-terminal hydrophobic signal sequence and transmembrane domain. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The canonical enzyme encoded by this gene is a lactosylceramide synthase important for glycolipid biosynthesis. [provided by RefSeq, Jan 2020]
Transcription factors with Perturb-seq knockdown data for B4GALT6. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = B4GALT6 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of B4GALT6, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr18:31,497,250–31,498,794 | 187.8 kb | Distal (>10kb) Multiome | 737 | |
| chr18:31,681,283–31,681,462 | 4.4 kb | Proximal (<10kb) | 38 | |
| chr18:31,684,202–31,686,118 | 461 bp | At TSS Multiome | 718 | |
| chr18:31,686,804–31,687,500 | 968 bp | At TSS | 102 | |
| chr18:31,688,746–31,688,908 | 2.9 kb | Proximal (<10kb) | 43 | |
| chr18:31,825,470–31,827,414 | 141.0 kb | Distal (>10kb) Multiome HiCAR | 319 | |
| chr18:31,942,190–31,943,930 | 257.4 kb | Distal (>10kb) Multiome | 1005 | |
| chr18:31,946,388–31,947,273 | 261.0 kb | Distal (>10kb) Multiome | 158 |
Genomic view of the B4GALT6 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.