The Axin-related protein, Axin2, presumably plays an important role in the regulation of the stability of beta-catenin in the Wnt signaling pathway, like its rodent homologs, mouse conductin/rat axil. In mouse, conductin organizes a multiprotein complex of APC (adenomatous polyposis of the colon), beta-catenin, glycogen synthase kinase 3-beta, and conductin, which leads to the degradation of beta-catenin. Apparently, the deregulation of beta-catenin is an important event in the genesis of a number of malignancies. The AXIN2 gene has been mapped to 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Mutations in this gene have been associated with colorectal cancer with defective mismatch repair. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for AXIN2. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AXIN2 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AXIN2, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr17:65,294,030–65,294,715 | 266.8 kb | Distal (>10kb) Multiome | 350 | |
| chr17:65,495,039–65,495,613 | 65.8 kb | Distal (>10kb) Multiome | 288 | |
| chr17:65,550,282–65,550,589 | 9.7 kb | Proximal (<10kb) | 56 | |
| chr17:65,554,049–65,554,242 | 6.1 kb | Proximal (<10kb) | 44 | |
| chr17:65,556,478–65,556,900 | 3.4 kb | Proximal (<10kb) | 390 | |
| chr17:65,558,496–65,559,400 | 895 bp | At TSS | 236 | |
| chr17:65,559,817–65,562,359 | 835 bp | At TSS Multiome | 1015 | |
| chr17:65,565,001–65,565,977 | 4.7 kb | Proximal (<10kb) | 134 | |
| chr17:65,567,969–65,568,828 | 7.2 kb | Proximal (<10kb) Multiome | 137 | |
| chr17:65,572,070–65,573,554 | 11.6 kb | Distal (>10kb) Multiome | 475 | |
| chr17:65,576,866–65,577,684 | 16.0 kb | Distal (>10kb) Multiome | 140 |
Genomic view of the AXIN2 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.