This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The protein is a key component of a histone donor complex that functions in nucleosome assembly. It interacts with histones H3 and H4, and functions together with a chromatin assembly factor during DNA replication and repair. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for ASF1A. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ASF1A upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ASF1A, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:118,650,262–118,652,597 | 242.5 kb | Distal (>10kb) Multiome | 979 | |
| chr6:118,709,068–118,709,910 | 184.6 kb | Distal (>10kb) Multiome | 530 | |
| chr6:118,786,272–118,787,718 | 107.0 kb | Distal (>10kb) Multiome | 189 | |
| chr6:118,893,171–118,895,350 | 124 bp | At TSS Multiome | 1078 | |
| chr6:118,896,637–118,896,775 | 2.5 kb | Proximal (<10kb) | 273 | |
| chr6:118,903,566–118,904,607 | 9.8 kb | Proximal (<10kb) Multiome | 97 | |
| chr6:118,934,206–118,935,563 | 40.9 kb | Distal (>10kb) Multiome | 909 | |
| chr6:118,993,752–118,994,494 | 99.9 kb | Distal (>10kb) Multiome | 119 | |
| chr6:119,026,545–119,028,642 | 133.0 kb | Distal (>10kb) Multiome | 152 | |
| chr6:119,034,732–119,036,201 | 141.4 kb | Distal (>10kb) Multiome | 248 | |
| chr6:119,037,796–119,038,720 | 144.1 kb | Distal (>10kb) Multiome | 230 | |
| chr6:119,077,736–119,079,370 | 184.7 kb | Distal (>10kb) Multiome HiCAR | 903 |
Genomic view of the ASF1A locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.