This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active (GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomain protein that functions to promote Erk activation and cell motility. Alternative splicing results in multiple transcript variants. Read-through transcripts from the upstream proline rich 5, renal (PRR5) gene into this gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]
Transcription factors with Perturb-seq knockdown data for ARHGAP8. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARHGAP8 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARHGAP8, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr22:44,497,886–44,498,569 | 254.3 kb | Distal (>10kb) Multiome | 437 | |
| chr22:44,661,031–44,661,516 | 91.4 kb | Distal (>10kb) Multiome | 28 | |
| chr22:44,668,163–44,669,181 | 84.0 kb | Distal (>10kb) Multiome | 265 | |
| chr22:44,684,755–44,685,227 | 67.6 kb | Distal (>10kb) Multiome | 562 | |
| chr22:44,701,628–44,703,097 | 50.4 kb | Distal (>10kb) Multiome | 588 | |
| chr22:44,747,279–44,747,521 | 5.1 kb | Proximal (<10kb) | 52 | |
| chr22:44,752,293–44,752,996 | 11 bp | At TSS Multiome | 233 | |
| chr22:45,006,916–45,010,513 | 257.5 kb | Distal (>10kb) Multiome | 522 | |
| chr22:45,052,140–45,053,598 | 300.4 kb | Distal (>10kb) Multiome | 132 |
Genomic view of the ARHGAP8 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.