This gene is a member of the human ADP-ribosylation factor (ARF) gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. [provided by RefSeq, Dec 2010]
Transcription factors with Perturb-seq knockdown data for ARF5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = ARF5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of ARF5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr7:127,347,511–127,348,270 | 240.5 kb | Distal (>10kb) Multiome | 487 | |
| chr7:127,391,433–127,393,629 | 195.7 kb | Distal (>10kb) Multiome | 929 | |
| chr7:127,584,951–127,586,129 | 2.8 kb | Proximal (<10kb) Multiome | 808 | |
| chr7:127,587,636–127,589,370 | 23 bp | At TSS Multiome | 807 | |
| chr7:127,593,567–127,593,956 | 5.2 kb | Proximal (<10kb) | 36 | |
| chr7:127,598,211–127,598,642 | 9.8 kb | Proximal (<10kb) | 133 | |
| chr7:127,619,493–127,621,269 | 31.9 kb | Distal (>10kb) Multiome | 302 | |
| chr7:127,628,938–127,629,789 | 41.1 kb | Distal (>10kb) Multiome | 216 | |
| chr7:127,650,994–127,652,678 | 63.6 kb | Distal (>10kb) Multiome | 1039 | |
| chr7:127,665,882–127,666,444 | 77.7 kb | Distal (>10kb) Multiome | 161 |
Genomic view of the ARF5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.