API5

apoptosis inhibitor 5 | AAC-11, AAC11, API5L1

This gene encodes an apoptosis inhibitory protein whose expression prevents apoptosis after growth factor deprivation. This protein suppresses the transcription factor E2F1-induced apoptosis and also interacts with, and negatively regulates Acinus, a nuclear factor involved in apoptotic DNA fragmentation. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Member of: DE-5 DE-5.21 Developmental clusters: GC1

Biological processes 19 terms

RNA binding (GO:0003723)RNA binding (GO:0003723)cytoplasm (GO:0005737)cytoplasm (GO:0005737)fibroblast growth factor binding (GO:0017134)membrane (GO:0016020)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic process (GO:0043066)negative regulation of apoptotic process (GO:0043066)nuclear speck (GO:0016607)nucleus (GO:0005634)nucleus (GO:0005634)nucleus (GO:0005634)protein binding (GO:0005515)spliceosomal complex (GO:0005681)spliceosomal complex (GO:0005681)

Expression (TPM)

TFs regulating API5 0 TFs

Transcription factors with Perturb-seq knockdown data for API5. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = API5 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.

TF	Mean coef	Binding	Outlier	TF→Gene link

Rows:

Elements linked to API5

Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of API5, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.

Element	Dist. to TSS	Link type	TFs
chr11:43,074,508–43,075,922	236.7 kb	Distal (>10kb) Multiome	328
chr11:43,118,911–43,119,871	192.5 kb	Distal (>10kb) Multiome	87
chr11:43,130,136–43,131,188	181.2 kb	Distal (>10kb) Multiome	99
chr11:43,311,481–43,312,563	38 bp	At TSS Multiome	920
chr11:43,358,425–43,359,827	47.0 kb	Distal (>10kb) Multiome	931
chr11:43,576,840–43,577,310	265.2 kb	Distal (>10kb) Multiome	464
chr11:43,578,930–43,580,292	267.4 kb	Distal (>10kb) Multiome	326

Genome Browser

Genomic view of the API5 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.