This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers, where disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jun 2022]
Transcription factors with Perturb-seq knockdown data for APC. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = APC upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of APC, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr5:112,417,921–112,420,785 | 289.5 kb | Distal (>10kb) Multiome | 740 | |
| chr5:112,707,010–112,708,233 | 40 bp | At TSS Multiome | 823 | |
| chr5:112,710,793–112,712,214 | 3.8 kb | Proximal (<10kb) Multiome | 154 | |
| chr5:112,717,567–112,717,768 | 10.0 kb | Proximal (<10kb) | 26 | |
| chr5:112,737,252–112,737,396 | 487 bp | At TSS | 42 | |
| chr5:112,737,494–112,738,559 | 30.2 kb | Distal (>10kb) Multiome | 697 | |
| chr5:112,848,068–112,848,715 | 140.9 kb | Distal (>10kb) Multiome | 275 | |
| chr5:112,860,721–112,861,653 | 153.7 kb | Distal (>10kb) Multiome | 948 | |
| chr5:112,920,841–112,922,670 | 213.7 kb | Distal (>10kb) Multiome | 913 | |
| chr5:112,976,029–112,977,339 | 269.1 kb | Distal (>10kb) Multiome | 889 |
Genomic view of the APC locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.