This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2015]
Transcription factors with Perturb-seq knockdown data for AP2M1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AP2M1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AP2M1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr3:183,883,494–183,884,017 | 291.3 kb | Distal (>10kb) Multiome | 83 | |
| chr3:183,884,110–183,885,311 | 289.9 kb | Distal (>10kb) Multiome | 972 | |
| chr3:183,912,940–183,913,709 | 261.6 kb | Distal (>10kb) Multiome | 225 | |
| chr3:184,017,103–184,018,456 | 157.0 kb | Distal (>10kb) Multiome | 878 | |
| chr3:184,134,086–184,134,568 | 40.7 kb | Distal (>10kb) Multiome | 732 | |
| chr3:184,134,801–184,135,887 | 39.5 kb | Distal (>10kb) Multiome | 941 | |
| chr3:184,154,967–184,155,880 | 19.6 kb | Distal (>10kb) Multiome | 580 | |
| chr3:184,174,432–184,175,524 | 110 bp | At TSS Multiome | 765 | |
| chr3:184,176,129–184,177,706 | 1.8 kb | Proximal (<10kb) Multiome | 480 | |
| chr3:184,185,411–184,187,065 | 11.1 kb | Distal (>10kb) Multiome | 878 | |
| chr3:184,229,229–184,229,859 | 54.6 kb | Distal (>10kb) Multiome | 409 | |
| chr3:184,236,026–184,236,474 | 61.3 kb | Distal (>10kb) Multiome | 41 | |
| chr3:184,241,140–184,242,189 | 66.9 kb | Distal (>10kb) Multiome HiCAR | 700 | |
| chr3:184,248,652–184,250,567 | 74.8 kb | Distal (>10kb) Multiome HiCAR | 1061 | |
| chr3:184,259,723–184,260,497 | 85.2 kb | Distal (>10kb) Multiome | 318 | |
| chr3:184,260,815–184,262,571 | 86.8 kb | Distal (>10kb) Multiome | 511 | |
| chr3:184,298,591–184,299,718 | 124.2 kb | Distal (>10kb) Multiome | 768 | |
| chr3:184,314,169–184,315,514 | 139.7 kb | Distal (>10kb) Multiome | 761 | |
| chr3:184,335,265–184,337,276 | 161.1 kb | Distal (>10kb) Multiome | 796 | |
| chr3:184,338,471–184,338,964 | 163.8 kb | Distal (>10kb) Multiome HiCAR | 203 | |
| chr3:184,361,172–184,363,709 | 188.3 kb | Distal (>10kb) Multiome | 1034 | |
| chr3:184,379,849–184,380,935 | 205.3 kb | Distal (>10kb) Multiome | 242 |
Genomic view of the AP2M1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.