Enables enzyme binding activity; protein phosphatase activator activity; and ubiquitin-like ligase-substrate adaptor activity. Involved in several processes, including macroautophagy; positive regulation of free ubiquitin chain polymerization; and positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction. Located in cytosol. Part of Cul4-RING E3 ubiquitin ligase complex. Is active in cytoskeleton; mitochondrion; and nucleus. Biomarker of multiple system atrophy. [provided by Alliance of Genome Resources, Jul 2025]
Transcription factors with Perturb-seq knockdown data for AMBRA1. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AMBRA1 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AMBRA1, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr11:46,294,934–46,295,998 | 298.4 kb | Distal (>10kb) Multiome | 533 | |
| chr11:46,296,747–46,297,460 | 296.9 kb | Distal (>10kb) Multiome | 415 | |
| chr11:46,338,698–46,339,679 | 254.8 kb | Distal (>10kb) Multiome | 393 | |
| chr11:46,346,987–46,348,092 | 246.7 kb | Distal (>10kb) Multiome | 586 | |
| chr11:46,352,002–46,352,557 | 241.7 kb | Distal (>10kb) Multiome | 306 | |
| chr11:46,379,120–46,383,444 | 214.4 kb | Distal (>10kb) Multiome | 930 | |
| chr11:46,388,459–46,390,750 | 205.2 kb | Distal (>10kb) Multiome | 593 | |
| chr11:46,391,139–46,393,221 | 201.3 kb | Distal (>10kb) Multiome | 965 | |
| chr11:46,407,311–46,408,466 | 186.2 kb | Distal (>10kb) Multiome | 157 | |
| chr11:46,554,280–46,555,184 | 39.3 kb | Distal (>10kb) Multiome | 531 | |
| chr11:46,593,562–46,594,345 | 45 bp | At TSS Multiome | 845 | |
| chr11:46,616,855–46,618,064 | 23.3 kb | Distal (>10kb) Multiome | 823 | |
| chr11:46,664,502–46,665,016 | 70.7 kb | Distal (>10kb) Multiome | 126 | |
| chr11:46,674,791–46,675,556 | 81.1 kb | Distal (>10kb) Multiome | 308 | |
| chr11:46,700,262–46,701,275 | 106.7 kb | Distal (>10kb) Multiome | 997 | |
| chr11:46,826,562–46,827,053 | 232.9 kb | Distal (>10kb) Multiome | 529 | |
| chr11:46,845,538–46,846,680 | 252.2 kb | Distal (>10kb) Multiome | 879 |
Genomic view of the AMBRA1 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.