The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells. It associates with protein kinases A and C and phosphatase, and serves as a scaffold protein in signal transduction. This protein and RII PKA colocalize at the cell periphery. This protein is a cell growth-related protein. Antibodies to this protein can be produced by patients with myasthenia gravis. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for AKAP12. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AKAP12 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AKAP12, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr6:151,045,533–151,045,907 | 194.8 kb | Distal (>10kb) Multiome | 88 | |
| chr6:151,239,598–151,241,662 | 102 bp | At TSS Multiome | 604 | |
| chr6:151,373,738–151,374,525 | 133.8 kb | Distal (>10kb) Multiome | 304 | |
| chr6:151,389,414–151,392,227 | 150.1 kb | Distal (>10kb) Multiome | 1234 | |
| chr6:151,451,713–151,453,312 | 211.9 kb | Distal (>10kb) Multiome | 1109 | |
| chr6:151,456,653–151,457,328 | 216.7 kb | Distal (>10kb) Multiome | 65 | |
| chr6:151,493,661–151,494,985 | 253.8 kb | Distal (>10kb) Multiome | 691 |
Genomic view of the AKAP12 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.