This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for AK4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AK4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AK4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:63,574,025–63,574,937 | 1573.2 kb | Distal (>10kb) Multiome HiCAR | 44 | |
| chr1:64,965,270–64,967,021 | 181.1 kb | Distal (>10kb) Multiome | 764 | |
| chr1:65,002,269–65,003,167 | 144.9 kb | Distal (>10kb) Multiome | 584 | |
| chr1:65,065,952–65,068,343 | 81.1 kb | Distal (>10kb) Multiome | 865 | |
| chr1:65,139,896–65,140,272 | 7.4 kb | Proximal (<10kb) | 15 | |
| chr1:65,147,108–65,150,062 | 93 bp | At TSS Multiome | 912 | |
| chr1:65,150,597–65,151,062 | 2.9 kb | Proximal (<10kb) | 93 | |
| chr1:65,253,859–65,255,712 | 106.8 kb | Distal (>10kb) Multiome | 756 | |
| chr1:65,265,365–65,266,424 | 118.2 kb | Distal (>10kb) Multiome | 602 | |
| chr1:65,308,757–65,310,566 | 162.3 kb | Distal (>10kb) Multiome | 950 | |
| chr1:65,419,304–65,421,455 | 272.7 kb | Distal (>10kb) Multiome | 961 | |
| chr1:66,923,845–66,926,268 | 1777.5 kb | Distal (>10kb) Multiome HiCAR | 945 | |
| chr1:66,929,624–66,931,089 | 1782.6 kb | Distal (>10kb) Multiome HiCAR | 922 |
Genomic view of the AK4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.