This gene encodes a member of the Argonaute family of proteins which contain PAZ and PIWI domains and play an integral role in RNA interference and short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of related family members. [provided by RefSeq, Mar 2017]
Transcription factors with Perturb-seq knockdown data for AGO4. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AGO4 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AGO4, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:35,533,667–35,534,085 | 274.1 kb | Distal (>10kb) Multiome | 64 | |
| chr1:35,556,848–35,558,023 | 250.4 kb | Distal (>10kb) Multiome | 894 | |
| chr1:35,573,081–35,573,635 | 234.6 kb | Distal (>10kb) Multiome | 174 | |
| chr1:35,577,045–35,577,930 | 230.4 kb | Distal (>10kb) Multiome | 235 | |
| chr1:35,641,114–35,641,872 | 166.4 kb | Distal (>10kb) Multiome | 824 | |
| chr1:35,707,208–35,708,693 | 100.1 kb | Distal (>10kb) Multiome | 388 | |
| chr1:35,717,979–35,719,281 | 89.1 kb | Distal (>10kb) Multiome | 558 | |
| chr1:35,769,208–35,770,406 | 38.0 kb | Distal (>10kb) Multiome | 929 | |
| chr1:35,807,537–35,808,824 | 36 bp | At TSS Multiome | 575 | |
| chr1:35,882,591–35,884,430 | 75.1 kb | Distal (>10kb) Multiome | 802 | |
| chr1:35,885,650–35,886,507 | 78.1 kb | Distal (>10kb) Multiome | 156 | |
| chr1:35,930,610–35,931,886 | 123.0 kb | Distal (>10kb) Multiome | 903 | |
| chr1:36,083,788–36,084,510 | 276.1 kb | Distal (>10kb) Multiome | 194 | |
| chr1:36,088,326–36,089,393 | 280.9 kb | Distal (>10kb) Multiome | 1023 |
Genomic view of the AGO4 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.