This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Transcription factors with Perturb-seq knockdown data for AGO3. The Binding column indicates whether any binding evidence exists for this TF–gene pair (ChIP-seq or motif footprint peaks). The Mean coef is the average Perturb-seq regression coefficient across active gRNAs (positive = AGO3 upregulated upon KD; negative = downregulated). The Outlier column indicates whether this gene is in the top or bottom 5% of all TF knockdown effects.
| TF | Mean coef | Binding | Outlier | TF→Gene link |
|---|
Open chromatin peaks (ATAC-seq) in the genomic neighbourhood of AGO3, linked by TSS proximity or chromatin conformation (Multiome / HiCAR). Each element overlaps at least one TF ChIP-seq binding site — the TFs column shows how many distinct TFs bind that element.
| Accessibility | Element | Dist. to TSS | Link type | TFs |
|---|---|---|---|---|
| chr1:35,641,114–35,641,872 | 289.7 kb | Distal (>10kb) Multiome | 824 | |
| chr1:35,707,208–35,708,693 | 223.3 kb | Distal (>10kb) Multiome | 388 | |
| chr1:35,717,979–35,719,281 | 212.3 kb | Distal (>10kb) Multiome | 558 | |
| chr1:35,769,208–35,770,406 | 161.3 kb | Distal (>10kb) Multiome | 929 | |
| chr1:35,807,537–35,808,824 | 123.2 kb | Distal (>10kb) Multiome | 575 | |
| chr1:35,882,591–35,884,430 | 48.1 kb | Distal (>10kb) Multiome | 802 | |
| chr1:35,885,650–35,886,507 | 45.1 kb | Distal (>10kb) Multiome | 156 | |
| chr1:35,930,610–35,931,886 | 247 bp | At TSS Multiome | 903 | |
| chr1:36,083,788–36,084,510 | 152.9 kb | Distal (>10kb) Multiome | 194 | |
| chr1:36,088,326–36,089,393 | 157.6 kb | Distal (>10kb) Multiome | 1023 | |
| chr1:36,148,864–36,150,075 | 218.3 kb | Distal (>10kb) Multiome | 925 | |
| chr1:36,150,344–36,151,320 | 219.8 kb | Distal (>10kb) Multiome | 524 | |
| chr1:36,155,033–36,157,490 | 224.7 kb | Distal (>10kb) Multiome | 790 | |
| chr1:36,166,478–36,167,190 | 235.6 kb | Distal (>10kb) Multiome | 85 | |
| chr1:36,176,148–36,176,823 | 245.2 kb | Distal (>10kb) Multiome | 141 | |
| chr1:36,178,119–36,179,461 | 247.8 kb | Distal (>10kb) Multiome | 222 | |
| chr1:36,187,827–36,188,464 | 256.9 kb | Distal (>10kb) Multiome | 572 | |
| chr1:36,223,459–36,225,047 | 293.1 kb | Distal (>10kb) Multiome | 1017 |
Genomic view of the AGO3 locus showing ATAC-seq accessibility and RNA-seq expression across the ESC → DE time course, together with TF binding peaks and element-to-TSS loop connections.